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Protein A and Protein G are recombinant proteins of microbial origin with the ability to bind mammalian immunoglobulin molecules. PureBiotech LLC's highly purified form of these proteins is covalently coupled to our magnetic particles (MPG™) to provide the quickest and easiest small scale method for purifying antibodies. The interaction between the proteins and IgG is not equivalent for all species or all antibody subclasses. The tables below will help you decide which Magnetic Affinity Support is best for your application.
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|
| Source |
E. coli |
E. coli |
| Molecular Weight |
42,000 |
22,600 |
| Number of Binding Sites for IgG |
5 |
2 |
| Albumin Binding Site |
No |
No |
| Optimal Binding pH |
8.2 |
5 |
|
| BINDING CAPABILITIES OF IMMUNOGLOBULIN PROTEINS |
| Antibody |
Protein A |
Protein G |
|
| Human IgG |
S |
S |
| Human IgG1 |
S |
S |
| Human IgG2 |
S |
S |
| Human IgG3 |
W |
S |
| Human IgG4 |
S |
S |
| Human IgA |
W |
NB |
| Human IgA1 |
W |
NB |
| Human IgA2 |
W |
NB |
| Human IgM |
W |
NB |
| Human IgE |
M |
NB |
| Human IgD |
NB |
NB |
| Mouse IgG |
S |
S |
| Mouse IgG1 |
W |
M |
| Mouse IgG2a |
S |
S |
| Mouse IgG2b |
S |
S |
| Mouse IgG3 |
S |
S |
| Mouse IgGM |
NB |
NB |
| Rat IgG |
W |
M |
| Rat IgG1 |
W |
M |
| Rat IgG2a |
NB |
S |
| Rat IgG2b |
NB |
W |
| Rat IgG2c |
S |
S |
| Rat IgG1 |
NB |
NB |
| Rabbit IgG |
S |
S |
| Rabbit IgM |
NB |
NB |
| Goat IgG |
W |
S |
| Goat IgG1 |
W |
S |
| Goat IgG2 |
S |
S |
| Goat IgM |
NB |
NB |
| Sheep IgG |
W |
S |
| Sheep IgG1 |
W |
S |
| Sheep IgG2 |
S |
S |
| Sheep IgM |
NB |
NB |
| Bovine IgG |
W |
S |
| Bovine IgG1 |
W |
S |
| Bovine IgG2 |
S |
S |
| Horse IgG |
W |
S |
| Guinea Pig IgG |
S |
S |
| Guinea Pig IgG1 |
S |
S |
| Guinea Pig IgG2 |
S |
S |
| Hamster IgG |
M |
— |
| Pig IgG |
S |
W |
| Donkey IgG |
M |
S |
| Dog IgG |
S |
W |
| Cat IgG |
S |
W |
| Rhesus Monkey IgG |
S |
S |
| Chicken IgG |
NB |
NB |
W= weak binding; M=moderate binding; S=strong binding;
NB= no binding; — means not available |
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