Index: Volume 2 Number 1
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Rapid, Efficient and Easy-to-Use No Sample Dilution Sample Sizes Ranging From 20-50 µl Non-toxic Pre-packaged in a Variety of Pre-hydrated and Dry Formats

SPIN-Pure™ COLUMNS are specifically designed for the fast and efficient separation of large molecules from small molecules. These columns are economical, easy to use, fast and non-toxic. SPIN-Pure™ COLUMNS provide a superior alternative to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

Processing of samples is quick and easy. The SPIN-Pure™ COLUMNS are briefly spun to remove the interstitial fluid. The sample is then added and centrifuged through the column. SPIN-Pure™ COLUMNS remove contaminant molecules based on size exclusion chromatography. Molecules larger than the matrix pore size are excluded from the matrix and pass directly into the collection tube. Smaller molecules are retained within the column matrix. Greater than 98% of the contaminants are retained in the resin. After processing, your sample is free of contaminants and is recovered undiluted. Adsorption, washing, or special elution steps are not required and the fast 5 minute procedure gives you up to 90% or greater sample recovery.

SPIN-Pure™ COLUMNS are microcentrifuge compatible and can process sample volumes of 20 µl to 50 µl. The columns are supplied ready-to-use, pre-packed and pre-hydrated to save you time. They are packed with Sephadex® G-25 or Sephadex® G-50 size exclusion media and hydrated in your choice of DEPC-water for safe RNA purifications or with TE Buffer. SPIN-Pure™ COLUMNS are also available packed with dry Sephadex® G-25 or G-50 and can be hydrated with reagent-grade water or any suitable buffer prior to use. Each unit comes complete with wash and sample collection tubes.

The SPIN-Pure™ G-25 COLUMNS have an effective inclusion range for molecules of <700 Daltons and will provide excellent recovery of DNA fragments >10 bases. SPIN-Pure™ G-25 COLUMNS have been designed specifically for the following uses:

• Desalting

• Buffer Exchange

• Removal of low molecular weight precursors and contaminants

SPIN-Pure™ G-50 COLUMNS have an effective inclusion range for molecules of <10-20 KiloDaltons and provide excellent recovery of DNA fragments >20 bases. SPIN-Pure™ G-50 COLUMNS have been designed specifically for the following uses:

• Removal of unincorporated precursors in random-priming, nick-translation and end labeling reactions

• Removal of dye deoxyterminators in manual or automated sequencing reactions

• Purification of protein conjugates in iodination, cross-linking, fluorescence labeling, and haptenation

• Desalting/purification/buffer exchange of peptides or proteins prior to electrophoresis

Next Article: Avoid PCR Workstation Contamination