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APPLICATIONS
- Sequencing primers for CloneSure™ and other common vectors
- PCR primers for strand-specific biotinylation of cloned DNA
- Generation of single-strand probes/templates
These versatile oligonucleotides are provided in both biotinylated and non- biotinylated forms for use in solid phase and solution-based applications. These lyophilized primers are complementary to sequences found adjacent to the cloning sites of most commonly used cloning and expression vectors. They can be used in pairs to generate strand-specific biotinylated (or non-biotinylated) DNA containing the cloned sequences of interest. When used in conjunction with our MPGŪ DNA TempPrep™ Kit or our MPGŪ Streptavidin magnetic particles, the strands can be separated to prepare strand-specific solid phases for nucleic acid library panning and related applications, for solid phase sequencing, and for generating strand-specific probes.
| Product No. | Primer Description | Unit (nmoles) | Price | ||
|---|---|---|---|---|---|
| Bio- tinylated |
Non-Bio- tinylated |
Bio- tinylated |
Non-Bio- tinylated |
||
| P-LZFB | P-LZF | lac Z Forward | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(AAAGG GGGAT GTGCT GCAAG GCG)3' | |||||
| P-LZRB | P-LZR | lac Z Reverse | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(GCTTC CGGCT CGTAT GTTGT GTG)3' | |||||
| P-M13FB | P-M13F | M13 Forward (-35) | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(CCCAG TCACG ACGTT GTAAA ACG)3' | |||||
| P-M13RB | P-M13R | M13 Reverse (-45) | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(AGCGG ATAAC AATTT CACAC AGG)3' | |||||
| P-SK75B | P-SK75 | SK | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(CGCTC TAGAA CTAGT GGATC)3' | |||||
| P-KS75B | P-KS75 | KS | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(TCGAG GTCGA CGGTA TC)3' | |||||
| P-T375B | P-T375 | T3 | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(TAACC CTCAC TAAAG GGA)3' | |||||
| P-T775B | P-T775 | T7 | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(TAATA CGACT CACTA TAGGG)3' | |||||
| P-SP675B | P-SP675 | SP6 | 0.75 | $150.00 | $60.00 |
| (+/- 5' biotin) 5'd(ATTTA GGTGA CACTA TAG)3' | |||||
| MBOLG01 | — | Oligo (dT)25 | 8.0 | $100.00 | — |
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